I have seen Ghostbusters more times than I have seen any other movie. I quit counting at 100, and that was before I was 10 years old.
This scene, and the music with it, still fills me with dread. I've said it elsewhere before, but it's the beginning of the End (both of the world and of the movie). You just know the big showdown with the big boss is right around the corner.
As a side note, if you've ever heard the complete soundtrack to Ghostbusters, this song is weird as hell. This cool part is probably less than 1/5 of the whole song. The rest of it sounds like if Hall & Oates did the soundtrack to The Neverending Story.